Impact Assessment of vB_KpnP_K1-ULIP33 Bacteriophage on the Human Gut Microbiota Using a Dynamic In Vitro Model
Fanny Laforêt,
Céline Antoine,
Sarah Lebrun,
Irma Gonza,
Elizabeth Goya-Jorge,
Caroline Douny,
Jean-Noël Duprez,
Marie-Louise Scippo,
Bernard Taminiau,
Georges Daube,
Abdoulaye Fall,
Damien Thiry,
Véronique Delcenserie
Affiliations
Fanny Laforêt
Laboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Céline Antoine
Laboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Sarah Lebrun
Laboratory of Food Quality Management, Department of Food Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Irma Gonza
Laboratory of Food Quality Management, Department of Food Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Elizabeth Goya-Jorge
Laboratory of Food Quality Management, Department of Food Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Caroline Douny
Laboratory of Food Analysis, Department of Food Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Jean-Noël Duprez
Laboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Marie-Louise Scippo
Laboratory of Food Analysis, Department of Food Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Bernard Taminiau
Laboratory of Microbiology, Department of Food Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Georges Daube
Laboratory of Microbiology, Department of Food Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Abdoulaye Fall
FoodChain ID GENOMICS SA, En Hayeneux 62, 4040 Herstal, Belgium
Damien Thiry
Laboratory of Bacteriology, Department of Infectious and Parasitic Diseases, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
Véronique Delcenserie
Laboratory of Food Quality Management, Department of Food Sciences, FARAH, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium
New control methods are needed to counter antimicrobial resistances and the use of bacteriophages as an alternative treatment seems promising. To that end, the effect of the phage vB_KpnP_K1-ULIP33, whose host is the hypervirulent Klebsiella pneumoniae SA12 (ST23 and capsular type K1), was assessed on intestinal microbiota, using an in vitro model: the SHIME® system (Simulator of the Human Intestinal Microbial Ecosystem). After stabilization of the system, the phage was inoculated for 7 days and its persistence in the different colons was studied until its disappearance from the system. The concentration of short chain fatty acids in the colons showed good colonization of the bioreactors by the microbiota and no significant effect related to the phage treatment. Diversity (α and β), the relative abundance of bacteria, and qPCR analysis targeting different genera of interest showed no significant variation following phage administration. Even if further in vitro studies are needed to assess the efficacy of this phage against its bacterial host within the human intestinal ecosystem, the phage ULIP33 exerted no significant change on the global colonic microbiota.
