A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection

Abstract

Summary: Here, we present a protocol to characterize zebrafish LGP2 as a dual regulator of interferon (IFN) response. We detail in vivo assays using time-lapse comparison of IFN response between wild-type and lgp2 knockout zebrafish following spring viraemia of carp virus (SVCV) infection. We also describe in vitro assays including titration of infection duration in SVCV-infected fish cells to determine changes in IFN response. This protocol is effective to illuminate a regulatory switch of LGP2 in fish cells toward virus infection.For complete details on the use and execution of this protocol, please refer to Gong et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Immunology
• Model Organisms
• Molecular Biology
• Signal Transduction
• Protein Biochemistry