Phototherapy Effect of Multifunctional Nano-cerium Oxide Quantum Dots on HepG2 Cells

Abstract

Objective To investigate the phototherapy effect of multifunctional nanometer cerium oxide quantum dot (HACQDs-Ce6) on HepG2 cells. Methods The dissolved oxygen analyzer was used to detect the catalytic oxygen production capacity of HACQDs-Ce6. CCK-8 method, scratch test and Calcein AM/PI staining were used to analyze the effects of HACQDs-Ce6 on the proliferation, migration and apoptosis of HepG2 cells. The recombinant mouse macrophage-granulocyte colony stimulating factor (rm GM-CSF) and recombinant mouse interleukin-4 (rm IL-4) were used to induce the differentiation of mouse bone marrow precursor cells into dendritic cells (DC) in vitro to investigate the effect of HACQDs-Ce6 on DC cells. Results HACQDs-Ce6 had catalytic oxygen production capacity. The proliferation activity of cells in the laser group was significantly lower than that in the dark group when the concentration of HACQDs-Ce6 was greater than 0.125 μg/ml (P=0.018). The migration ability of HepG2 cells in HACQDs-Ce6 group was significantly lower than that in the PBS group (P < 0.01). HACQDs-Ce6 almost had no effect on HFF-1/L wnt-3A and DC cells. Calcein AM/PI staining showed that the combined action of HACQDs-Ce6 and PDT killed almost all the HepG2 cells. Conclusion The phototherapy effect of HACQDs-Ce6 on HepG2 cells is obvious, with no damage to HFF-1/L wnt-3A/DC cells.

Keywords

• nanomaterials
• tumor
• photodynamic therapy
• dendritic cell