Nature Communications (Jan 2020)

Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing

  • Kailong Li,
  • Yuxuan Liu,
  • Hui Cao,
  • Yuannyu Zhang,
  • Zhimin Gu,
  • Xin Liu,
  • Andy Yu,
  • Pranita Kaphle,
  • Kathryn E. Dickerson,
  • Min Ni,
  • Jian Xu

DOI
https://doi.org/10.1038/s41467-020-14362-5
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 16

Abstract

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Tissues-specific gene expression requires coordinated cis-regulatory elements. Here the authors use dCas9-based enhancer targeting to remodel local epigenetic landscapes and activate or inactive transcription.