Nature Communications (Apr 2023)
Workflow enabling deepscale immunopeptidome, proteome, ubiquitylome, phosphoproteome, and acetylome analyses of sample-limited tissues
- Jennifer G. Abelin,
- Erik J. Bergstrom,
- Keith D. Rivera,
- Hannah B. Taylor,
- Susan Klaeger,
- Charles Xu,
- Eva K. Verzani,
- C. Jackson White,
- Hilina B. Woldemichael,
- Maya Virshup,
- Meagan E. Olive,
- Myranda Maynard,
- Stephanie A. Vartany,
- Joseph D. Allen,
- Kshiti Phulphagar,
- M. Harry Kane,
- Suzanna Rachimi,
- D. R. Mani,
- Michael A. Gillette,
- Shankha Satpathy,
- Karl R. Clauser,
- Namrata D. Udeshi,
- Steven A. Carr
Affiliations
- Jennifer G. Abelin
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Erik J. Bergstrom
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Keith D. Rivera
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Hannah B. Taylor
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Susan Klaeger
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Charles Xu
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Eva K. Verzani
- Broad Institute of Massachusetts Institute of Technology and Harvard
- C. Jackson White
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Hilina B. Woldemichael
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Maya Virshup
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Meagan E. Olive
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Myranda Maynard
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Stephanie A. Vartany
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Joseph D. Allen
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Kshiti Phulphagar
- Broad Institute of Massachusetts Institute of Technology and Harvard
- M. Harry Kane
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Suzanna Rachimi
- Broad Institute of Massachusetts Institute of Technology and Harvard
- D. R. Mani
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Michael A. Gillette
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Shankha Satpathy
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Karl R. Clauser
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Namrata D. Udeshi
- Broad Institute of Massachusetts Institute of Technology and Harvard
- Steven A. Carr
- Broad Institute of Massachusetts Institute of Technology and Harvard
- DOI
- https://doi.org/10.1038/s41467-023-37547-0
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 22
Abstract
Patient samples are often available in limited amounts, restricting the number of possible omics analyses. Here the authors present MONTE, a workflow that enables serial HLA-I and HLA-II immunopeptidome, ubiquitylome, proteome, phosphoproteome, and acetylome data collection from patient samples.
