Accelerated Metabolite Levels of Aerobic Glycolysis and the Pentose Phosphate Pathway Are Required for Efficient Replication of Infectious Spleen and Kidney Necrosis Virus in Chinese Perch Brain Cells
Xixi Guo,
Shiwei Wu,
Ningqiu Li,
Qiang Lin,
Lihui Liu,
Hongru Liang,
Yinjie Niu,
Zhibin Huang,
Xiaozhe Fu
Affiliations
Xixi Guo
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou 510380, China
Shiwei Wu
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou 510380, China
Ningqiu Li
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou 510380, China
Qiang Lin
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou 510380, China
Lihui Liu
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou 510380, China
Hongru Liang
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou 510380, China
Yinjie Niu
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou 510380, China
Zhibin Huang
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou 510380, China
Xiaozhe Fu
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou 510380, China
Glucose is a main carbon and energy source for virus proliferation and is usually involved in the glycolysis, pentose phosphate pathway (PPP), and tricarboxylic acid cycle (TCA cycle) pathways. In this study, we investigated the roles of glucose-related metabolic pathways during the replication of infectious spleen and kidney necrosis virus (ISKNV), which has caused serious economic losses in the cultured Chinese perch (Siniperca chuatsi) industry. We found that ISKNV infection enhanced the metabolic pathways of the PPP and the TCA cycle at the early stage of the ISKNV infection cycle and enhanced the glycolysis pathway at the late stage of the ISKNV infection cycle though the comprehensive analysis of transcriptomics, proteomics, and metabolomics. The advanced results proved that ISKNV replication induced upregulation of aerobic glycolysis at the late stage of ISKNV infection cycle and aerobic glycolysis were required for ISKNV multiplication. In addition, the PPP, providing nucleotide biosynthesis, was also required for ISKNV multiplication. However, the TCA cycle involving glucose was not important and necessary for ISKNV multiplication. The results reported here provide new insights into viral pathogenesis mechanism of metabolic shift, as well as antiviral treatment strategies.
