P-TDHM: Open-source portable telecentric digital holographic microscope
Lei Jin,
Ziyang Yu,
Aaron Au,
Peter Serles,
Nan Wang,
Jeremy T. Lant,
Tobin Filleter,
Christopher M. Yip
Affiliations
Lei Jin
Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada; Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada
Ziyang Yu
Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada; Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada
Aaron Au
Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada; Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada
Peter Serles
Department of Mechanical and Industrial Engineering, 5 King's College Road, Toronto, ON M5S 3G8, Canada
Nan Wang
Civil and Environmental Engineering, 527 College Avenue, Cornell University, Ithaca, NY 14853, United States
Jeremy T. Lant
Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada; Department of Chemistry, 80 St. George Street, Toronto, ON M5S 3H6, Canada
Tobin Filleter
Department of Mechanical and Industrial Engineering, 5 King's College Road, Toronto, ON M5S 3G8, Canada
Christopher M. Yip
Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada; Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada; Department of Chemical Engineering & Applied Chemistry, 200 College St, Toronto, ON M5S 3E5, Canada; Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada; Corresponding author at: Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada.
We present the design of a low-cost, portable telecentric digital holographic microscope (P-TDHM) that utilizes off-the-shelf components. We describe the system’s hardware and software elements and evaluate its performance by imaging samples ranging from nano-printed targets to live HeLa cells, HEK293 cells, and Dolichospermum via both in-line and off-axis modes. Our results demonstrate that the system can acquire high quality quantitative phase images with nanometer axial and sub-micron lateral resolution in a small form factor, making it a promising candidate for resource-limited settings and remote locations. Our design represents a significant step forward in making telecentric digital holographic microscopy accessible and affordable to the broader community.
