P-TDHM: Open-source portable telecentric digital holographic microscope

Lei Jin; Ziyang Yu; Aaron Au; Peter Serles; Nan Wang; Jeremy T. Lant; Tobin Filleter; Christopher M. Yip

HardwareX (Mar 2024)

P-TDHM: Open-source portable telecentric digital holographic microscope

Lei Jin,
Ziyang Yu,
Aaron Au,
Peter Serles,
Nan Wang,
Jeremy T. Lant,
Tobin Filleter,
Christopher M. Yip

Affiliations

Lei Jin: Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada; Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada
Ziyang Yu: Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada; Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada
Aaron Au: Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada; Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada
Peter Serles: Department of Mechanical and Industrial Engineering, 5 King's College Road, Toronto, ON M5S 3G8, Canada
Nan Wang: Civil and Environmental Engineering, 527 College Avenue, Cornell University, Ithaca, NY 14853, United States
Jeremy T. Lant: Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada; Department of Chemistry, 80 St. George Street, Toronto, ON M5S 3H6, Canada
Tobin Filleter: Department of Mechanical and Industrial Engineering, 5 King's College Road, Toronto, ON M5S 3G8, Canada
Christopher M. Yip: Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada; Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada; Department of Chemical Engineering & Applied Chemistry, 200 College St, Toronto, ON M5S 3E5, Canada; Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada; Corresponding author at: Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada.

Journal volume & issue: Vol. 17
p. e00508

Abstract

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We present the design of a low-cost, portable telecentric digital holographic microscope (P-TDHM) that utilizes off-the-shelf components. We describe the system’s hardware and software elements and evaluate its performance by imaging samples ranging from nano-printed targets to live HeLa cells, HEK293 cells, and Dolichospermum via both in-line and off-axis modes. Our results demonstrate that the system can acquire high quality quantitative phase images with nanometer axial and sub-micron lateral resolution in a small form factor, making it a promising candidate for resource-limited settings and remote locations. Our design represents a significant step forward in making telecentric digital holographic microscopy accessible and affordable to the broader community.

Published in HardwareX

ISSN: 2468-0672 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Science (General)
Website: https://www.journals.elsevier.com/hardwarex/

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Abstract

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