Identification and characterization of interacting proteins of transcription factor DpWRI1-like related to lipid biosynthesis from microalga Dunaliella parva
Lingru Ruan,
Limei Huang,
Lina Wu,
Jinghui Gu,
Yanyan Liang,
Xiuli Liang,
Changhua Shang
Affiliations
Lingru Ruan
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, Guangxi, 541006, China
Limei Huang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, Guangxi, 541006, China
Lina Wu
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, Guangxi, 541006, China
Jinghui Gu
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, Guangxi, 541006, China
Yanyan Liang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, Guangxi, 541006, China
Xiuli Liang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, Guangxi, 541006, China
Changhua Shang
Corresponding author.; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University), University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, Guangxi, 541006, China
Our previous study found that Dunaliella parva WRINKLED1-like (DpWRI1-like) was a key regulatory factor of lipid biosynthesis in D. parva. DpWRI1-like gene and target genes of DpWRI1-like have been obtained in our previous study, but the interacting proteins of DpWRI1-like are unclear now, which has limited a deep understanding of the function of DpWRI1-like. Yeast two-hybrid was widely used to identify protein-protein interaction. In this study, the interacting proteins of DpWRI1-like were obtained using yeast two-hybrid technique to further realize the role of DpWRI1-like. Three important interacting proteins have the following predicted activities: acyl-CoA-binding domain-containing protein 6 (interacting protein 1, ACBD6), duplicated carbonic anhydrase (interacting protein 2, DCA) and DNA-binding transcription factor (interacting protein 3, TF). Bimolecular fluorescence complementation assay further validated the interaction between DpWRI1-like and interacting proteins ACBD6 and DCA. The further bioinformatics analyses of interacting proteins were conducted. Protein-protein docking indicated the strong affinity between DpWRI1-like and three interacting proteins. Since interacting proteins have been found to be related to lipid biosynthesis in other organisms, this study contributes to a deeper understanding of the role of DpWRI1-like in lipid synthesis. In conclusion, this study firstly reported three interacting proteins (ACBD6, DCA and TF) of DpWRI1-like related to lipid biosynthesis, and conducted their bioinformatics analyses, which would be conducive to a deep understanding of the function of DpWRI1-like in lipid biosynthesis.
