The roles and prognostic significance of ABI1-TSV-11 expression in patients with left-sided colorectal cancer

Yu Zhang; Zhaohui Zhong; Mei Li; Jingyi Chen; Tingru Lin; Jie Sun; Di Wang; Qing Mu; Huiting Su; Na Wu; Aiyu Liu; Yimeng Yu; Menglei Zhang; Yulan Liu; Jingzhu Guo; Weidong Yu

doi:10.1038/s41598-021-90220-8

Scientific Reports (May 2021)

The roles and prognostic significance of ABI1-TSV-11 expression in patients with left-sided colorectal cancer

Yu Zhang,
Zhaohui Zhong,
Mei Li,
Jingyi Chen,
Tingru Lin,
Jie Sun,
Di Wang,
Qing Mu,
Huiting Su,
Na Wu,
Aiyu Liu,
Yimeng Yu,
Menglei Zhang,
Yulan Liu,
Jingzhu Guo,
Weidong Yu

Affiliations

Yu Zhang: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Zhaohui Zhong: Department of General Surgery, Peking University People’s Hospital
Mei Li: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Jingyi Chen: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Tingru Lin: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Jie Sun: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Di Wang: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Qing Mu: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Huiting Su: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Na Wu: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Aiyu Liu: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Yimeng Yu: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital
Menglei Zhang: Department of Animal Laboratory, Peking University People’s Hospital
Yulan Liu: Department of Gastroenterology, Peking University People’s Hospital
Jingzhu Guo: Department of Pediatric, Peking University People’s Hospital
Weidong Yu: Department of Central Laboratory and Institute of Clinical Molecular Biology, Peking University People’s Hospital

DOI: https://doi.org/10.1038/s41598-021-90220-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 15

Abstract

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Abstract Abnormally expressed and/or phosphorylated Abelson interactor 1 (ABI1) participates in the metastasis and progression of colorectal cancer (CRC). ABI1 presents as at least 12 transcript variants (TSVs) by mRNA alternative splicing, but it is unknown which of them is involved in CRC metastasis and prognosis. Here, we firstly identified ABI1-TSV-11 as a key TSV affecting the metastasis and prognosis of left-sided colorectal cancer (LsCC) and its elevated expression is related to lymph node metastasis and shorter overall survival (OS) in LsCC by analyzing data from The Cancer Genome Atlas and TSVdb. Secondly, ABI1-TSV-11 overexpression promoted LoVo and SW480 cells adhesion and migration in vitro, and accelerated LoVo and SW480 cells lung metastasis in vivo. Finally, mechanism investigations revealed that ABI1-isoform-11 interacted with epidermal growth factor receptor pathway substrate 8 (ESP8) and regulated actin dynamics to affect LoVo and SW480 cells biological behaviors. Taken together, our data demonstrated that ABI1-TSV-11 plays an oncogenic role in LsCC, it is an independent risk factor of prognosis and may be a potential molecular marker and therapeutic target in LsCC.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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