Frontiers in Pharmacology (Jan 2021)
The Anti-Inflammatory Effect of KS23, A Novel Peptide Derived From Globular Adiponectin, on Endotoxin-Induced Uveitis in Rats
- Xin Shi,
- Xin Shi,
- Xin Shi,
- Xin Shi,
- Xin Shi,
- Shaopin Zhu,
- Shaopin Zhu,
- Shaopin Zhu,
- Shaopin Zhu,
- Shaopin Zhu,
- Huiyi Jin,
- Huiyi Jin,
- Huiyi Jin,
- Huiyi Jin,
- Huiyi Jin,
- Junwei Fang,
- Junwei Fang,
- Junwei Fang,
- Junwei Fang,
- Junwei Fang,
- Xindan Xing,
- Xindan Xing,
- Xindan Xing,
- Xindan Xing,
- Xindan Xing,
- Yihan Wang,
- Yihan Wang,
- Yihan Wang,
- Yihan Wang,
- Yihan Wang,
- Hanying Wang,
- Hanying Wang,
- Hanying Wang,
- Hanying Wang,
- Hanying Wang,
- Chingyi Wang,
- Chingyi Wang,
- Chingyi Wang,
- Chingyi Wang,
- Chingyi Wang,
- Tian Niu,
- Tian Niu,
- Tian Niu,
- Tian Niu,
- Tian Niu,
- Kun Liu,
- Kun Liu,
- Kun Liu,
- Kun Liu,
- Kun Liu
Affiliations
- Xin Shi
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Xin Shi
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Xin Shi
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Xin Shi
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Xin Shi
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- Shaopin Zhu
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Shaopin Zhu
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Shaopin Zhu
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Shaopin Zhu
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Shaopin Zhu
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- Huiyi Jin
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Huiyi Jin
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Huiyi Jin
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Huiyi Jin
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Huiyi Jin
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- Junwei Fang
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Junwei Fang
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Junwei Fang
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Junwei Fang
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Junwei Fang
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- Xindan Xing
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Xindan Xing
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Xindan Xing
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Xindan Xing
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Xindan Xing
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- Yihan Wang
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Yihan Wang
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Yihan Wang
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Yihan Wang
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Yihan Wang
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- Hanying Wang
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Hanying Wang
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Hanying Wang
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Hanying Wang
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Hanying Wang
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- Chingyi Wang
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Chingyi Wang
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Chingyi Wang
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Chingyi Wang
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Chingyi Wang
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- Tian Niu
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Tian Niu
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Tian Niu
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Tian Niu
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Tian Niu
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- Kun Liu
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Kun Liu
- National Clinical Research Center for Eye Diseases, Shanghai, China
- Kun Liu
- Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
- Kun Liu
- Shanghai Engineering Center for Visual Science and Photo Medicine, Shanghai, China
- Kun Liu
- Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
- DOI
- https://doi.org/10.3389/fphar.2020.585446
- Journal volume & issue
-
Vol. 11
Abstract
Purpose: Adiponectin has been shown to exert potent anti-inflammatory activities in a range of systemic inflammatory diseases. This study aimed to investigate the potential therapeutic effects of KS23, a globular adiponectin-derived peptide, on endotoxin-induced uveitis (EIU) in rats and lipopolysaccharide (LPS)-stimulated mouse macrophage-like RAW 264.7 cells.Methods: EIU was induced in Lewis rats by subcutaneous injection of LPS into a single footpad. KS23 or phosphate-buffered saline (PBS) was administered immediately after LPS induction via intravitreal injection. Twenty-four hours later, clinical and histopathological scores were evaluated, and the aqueous humor (AqH) was collected to determine the infiltrating cells, protein concentration, and levels of inflammatory cytokines. In vitro, cultured RAW 264.7 cells were stimulated with LPS in the presence or absence of KS23, inflammatory cytokine levels in the supernatant, nuclear translocation of nuclear factor kappa B (NF-κB) subunit p65, and the expression of NF-kB signaling pathway components were analyzed.Results: KS23 treatment significantly ameliorated the clinical and histopathological scores of EIU rats and reduced the levels of infiltration cells, protein, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the aqueous humor. Consistently, KS23 decreased the expression of TNF-α and IL-6 in the supernatant of LPS-stimulated RAW 264.7 cells and inhibited the LPS-induced nuclear translocation of NF-κB p65 and the phosphorylation of IKKα/β/IκBα/NF-κB.Conclusion: The in vivo and in vitro results demonstrated the anti-inflammatory effects of the peptide KS23 and suggested that KS23 is a compelling, novel therapeutic candidate for the treatment of ocular inflammation.
Keywords
- globular adiponectin
- endotoxin-induced uveitis
- inflammation
- nuclear factor kappa B
- lipopolysaccharide
