Frontiers in Cell and Developmental Biology (May 2022)
Transcriptomic Analysis Provides Insights to Reveal the bmp6 Function Related to the Development of Intermuscular Bones in Zebrafish
- Huan Xu,
- Huan Xu,
- Huan Xu,
- Huan Xu,
- Guangxiang Tong,
- Guangxiang Tong,
- Guangxiang Tong,
- Ting Yan,
- Ting Yan,
- Ting Yan,
- Le Dong,
- Le Dong,
- Xiaoxing Yang,
- Xiaoxing Yang,
- Dongyu Dou,
- Zhipeng Sun,
- Zhipeng Sun,
- Zhipeng Sun,
- Tianqi Liu,
- Tianqi Liu,
- Tianqi Liu,
- Xianhu Zheng,
- Xianhu Zheng,
- Xianhu Zheng,
- Jian Yang,
- Xiaowen Sun,
- Xiaowen Sun,
- Xiaowen Sun,
- Yi Zhou,
- Youyi Kuang,
- Youyi Kuang,
- Youyi Kuang
Affiliations
- Huan Xu
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Huan Xu
- National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, China
- Huan Xu
- Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Huan Xu
- Heilongjiang Provincial Key Laboratory of Hard Tissue Development and Regeneration, The Second Affiliated Hospital of Harbin Medical University, Harbin, China
- Guangxiang Tong
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Guangxiang Tong
- National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, China
- Guangxiang Tong
- Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Ting Yan
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Ting Yan
- National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, China
- Ting Yan
- Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Le Dong
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Le Dong
- College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China
- Xiaoxing Yang
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Xiaoxing Yang
- College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China
- Dongyu Dou
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Zhipeng Sun
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Zhipeng Sun
- National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, China
- Zhipeng Sun
- Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Tianqi Liu
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Tianqi Liu
- National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, China
- Tianqi Liu
- Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Xianhu Zheng
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Xianhu Zheng
- National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, China
- Xianhu Zheng
- Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Jian Yang
- Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo, China
- Xiaowen Sun
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Xiaowen Sun
- National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, China
- Xiaowen Sun
- Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Yi Zhou
- Stem Cell Program of Boston Children’s Hospital, Division of Hematology/Oncology, Boston Children’s Hospital and Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States
- Youyi Kuang
- Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Harbin, China
- Youyi Kuang
- National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Harbin, China
- Youyi Kuang
- Key Laboratory of Freshwater Aquatic Biotechnology and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- DOI
- https://doi.org/10.3389/fcell.2022.821471
- Journal volume & issue
-
Vol. 10
Abstract
Intermuscular bones (IBs) are small, hard-boned spicules located in the muscle tissue that mainly exist in the myosepta of lower teleosts, which hurt the edibleness and economic value of fish. The study of the development of IBs is very important for freshwater aquaculture fish, but the molecular mechanism of its formation and the key regulatory genes remain unclear. In this study, we first constructed two types of zebrafish mutants (the mutants losing IBs and the mutants with partial deletion of IBs) by knocking out bmp6. We then carried out a transcriptomic analysis to reveal the role of bmp6 in the developmental mechanism of IBs; we used the caudal musculoskeletal tissues of these mutants and wild-type zebrafish at three development stages (20, 45, and 60 dph) to perform transcriptomic analysis. The results showed that the deficiency of bmp6 upregulated sik1 and activated the TNF-A signaling via the NF-KB pathway, which inhibited the development of osteoblasts and promoted osteoclast formation, thereby inhibiting the formation of IBs. These results provided insights to understand the role of bmp6 in the development of IBs in zebrafish and are useful for selective breeding of IBs in cyprinids.
Keywords
