QTL Mapping for Seed Tocopherol Content in Soybean
Shibi Zhang,
Kwadwo Gyapong Agyenim-Boateng,
Shengrui Zhang,
Yongzhe Gu,
Jie Qi,
Muhammad Azam,
Caiyou Ma,
Yecheng Li,
Yue Feng,
Yitian Liu,
Jing Li,
Bin Li,
Lijuan Qiu,
Junming Sun
Affiliations
Shibi Zhang
College of Agronomy, Qingdao Agricultural University, Qingdao 266000, China
Kwadwo Gyapong Agyenim-Boateng
The National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Shengrui Zhang
The National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yongzhe Gu
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of Germplasm and Biotechnology (MARA), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jie Qi
The National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Muhammad Azam
The National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Caiyou Ma
The National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yecheng Li
The National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yue Feng
The National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yitian Liu
The National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jing Li
The National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Bin Li
MARA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Lijuan Qiu
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Key Laboratory of Germplasm and Biotechnology (MARA), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Junming Sun
College of Agronomy, Qingdao Agricultural University, Qingdao 266000, China
Tocopherol plays an important role as a powerful antioxidant in human beings and in plants. This study investigated the genetic basis of tocopherol content in soybean. A RIL population of 192 lines derived from 2 cultivars, ZH13 and ZH35, was evaluated for tocopherol content across 3 environments. QTL mapping identified 38 QTL for tocopherol, with stable QTL identified on Chromosomes 5 and 12. Ninety polymorphic genes were identified from these regions. Further SNP variation of a natural population identified 47 SNPs, with missense variants in 19 genes, including the heat shock transcription factor gene (GmHSFA8) and gamma-tocopherol methyltransferase (GmVTE4), potentially related to tocopherol accumulation in soybean. Haplotype analysis revealed significant variations in these missense variants in the natural population. This study provides insights into the genetic mechanisms underlying tocopherol content in soybean, which is important for breeding high tocopherol soybean cultivars.
