Detection of FY*A, FY*B and FY*X genotypes in Duffy blood group system by polymerase chain reaction-sequence specific primer (PCR-SSP

Abstract

Research problems: Serology method is the most commonly used for Duffy blood group phenotyping. This method is simple, convenient and inexpensive. However, mutation of FYx gene at position C265T can cause a weak expression of Fyb antigen and may not be detected by anti-Fyb which lead to false negative by serology testing. The objective of this study is to detect FY*A, FY*B and FY*X genotypes in Duffy blood group system by polymerase chain reaction-sequence specific primer (PCR-SSP). Materials and methods: Ninety seven samples were tused for phenotyping of Fya and Fyb by serology method and the DNA was extracted for detect FY*A and FY*B genotype by PCR-SSP. Results: Phenotyping of 97 samples showed that 78 samples (80.41%) were Fy(a+b-) and 19 samples (19.59%) were Fy(a+b). Seventy-eight DNA samples of Fy(a+b-) were then detected for FY*X genotype at position C265T by PCR-SSP. The results showed no C265T mutation in all tested samples. The comparison results between serology and PCR-SSP methods were concordant. Conclusions: PCR-SSP can be as alternative method to detect FY*A, FY*B and FY*X genotype in Duffy blood group system for selecting suitable blood to the patient who has anti-Fya and/or anti-Fyb in order to prevent adverse effects of transfusion reaction and investigate for the cause of hemolytic disease of the newborn. Bull Chiang Mai Assoc Med Sci 2015; 48(2): 136-143. Doi: 10.14456/jams.2015.11

Keywords

• Genotype
• FY*A
• FY*B
• FY*X
• Duffy
• PCR-SSP