Identification of a uroporphyrinogen III synthetase gene and characterization of its role in pearl sac formation in Hyriopsis cumingii

Jiexuan Shen; Dandan Huang; Jiale Li; Weixin Ye; Zheng Wang; Zhiyi Bai

Aquaculture and Fisheries (Jan 2022)

Identification of a uroporphyrinogen III synthetase gene and characterization of its role in pearl sac formation in Hyriopsis cumingii

Jiexuan Shen,
Dandan Huang,
Jiale Li,
Weixin Ye,
Zheng Wang,
Zhiyi Bai

Affiliations

Jiexuan Shen: Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Ministry of Agriculture, Shanghai, 201306, China
Dandan Huang: Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Ministry of Agriculture, Shanghai, 201306, China
Jiale Li: Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Ministry of Agriculture, Shanghai, 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, China
Weixin Ye: Zhexing Pearl Trading Co. Ltd, Zhejiang, 321001, China
Zheng Wang: Zhexing Pearl Trading Co. Ltd, Zhejiang, 321001, China
Zhiyi Bai: Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Ministry of Agriculture, Shanghai, 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, China; Corresponding author. Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Ministry of Agriculture, Shanghai, 201306, China.

Journal volume & issue: Vol. 7, no. 1
pp. 18 – 22

Abstract

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Uroporphyrinogen III synthetase (UROS) is an important enzyme involved in the structural formation of porphyrin, which is responsible for color intensity. A UROS homolog was isolated and characterized from the pearl mussel, Hyriopsis cumingii (HcUros). The HcUros cDNA was 858 bp in length with an open reading frame that encoded a 285 amino acid protein, which contained a conserved HemD domain. HcUros exhibited widespread tissue distribution, displaying particular enrichment in the mantle and haemolymph of purple mussels, compared to white mussels. In situ hybridization of HcUros in mantle tissue demonstrated that it was specifically expressed in the dorsal epithelial cells of the mantle pallia. Additionally, HcUros expression was upregulated following implantation of the pearl sac to produce pearls. The data suggest that HcUros contributes to shell pigmentation, and nacre formation and coloring in H. cumingii.

Published in Aquaculture and Fisheries

ISSN: 2468-550X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: https://www.keaipublishing.com/en/journals/aquaculture-and-fisheries

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