High-performance chemical- and light-inducible recombinases in mammalian cells and mice

Benjamin H. Weinberg; Jang Hwan Cho; Yash Agarwal; N. T. Hang Pham; Leidy D. Caraballo; Maciej Walkosz; Charina Ortega; Micaela Trexler; Nathan Tague; Billy Law; William K. J. Benman; Justin Letendre; Jacob Beal; Wilson W. Wong

doi:10.1038/s41467-019-12800-7

Nature Communications (Oct 2019)

High-performance chemical- and light-inducible recombinases in mammalian cells and mice

Benjamin H. Weinberg,
Jang Hwan Cho,
Yash Agarwal,
N. T. Hang Pham,
Leidy D. Caraballo,
Maciej Walkosz,
Charina Ortega,
Micaela Trexler,
Nathan Tague,
Billy Law,
William K. J. Benman,
Justin Letendre,
Jacob Beal,
Wilson W. Wong

Affiliations

Benjamin H. Weinberg: Department of Biomedical Engineering and Biological Design Center, Boston University
Jang Hwan Cho: Department of Biomedical Engineering and Biological Design Center, Boston University
Yash Agarwal: Department of Biomedical Engineering and Biological Design Center, Boston University
N. T. Hang Pham: Department of Biomedical Engineering and Biological Design Center, Boston University
Leidy D. Caraballo: Department of Biomedical Engineering and Biological Design Center, Boston University
Maciej Walkosz: Department of Biomedical Engineering and Biological Design Center, Boston University
Charina Ortega: Department of Biomedical Engineering and Biological Design Center, Boston University
Micaela Trexler: Department of Biomedical Engineering and Biological Design Center, Boston University
Nathan Tague: Department of Biomedical Engineering and Biological Design Center, Boston University
Billy Law: Department of Biomedical Engineering and Biological Design Center, Boston University
William K. J. Benman: Department of Biomedical Engineering and Biological Design Center, Boston University
Justin Letendre: Department of Biomedical Engineering and Biological Design Center, Boston University
Jacob Beal: Raytheon BBN Technologies
Wilson W. Wong: Department of Biomedical Engineering and Biological Design Center, Boston University

DOI: https://doi.org/10.1038/s41467-019-12800-7
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

Read online

The availability of high performance recombinases with low basal activity and high dynamic range is limited. Here the authors present a library of over 20 orthogonal split recombinases that can be induced by small molecules, light and temperature in vivo.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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