Nature Communications (Jun 2021)
A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses
- Alberto A. Amarilla,
- Julian D. J. Sng,
- Rhys Parry,
- Joshua M. Deerain,
- James R. Potter,
- Yin Xiang Setoh,
- Daniel J. Rawle,
- Thuy T. Le,
- Naphak Modhiran,
- Xiaohui Wang,
- Nias Y. G. Peng,
- Francisco J. Torres,
- Alyssa Pyke,
- Jessica J. Harrison,
- Morgan E. Freney,
- Benjamin Liang,
- Christopher L. D. McMillan,
- Stacey T. M. Cheung,
- Darwin J. Da Costa Guevara,
- Joshua M. Hardy,
- Mark Bettington,
- David A. Muller,
- Fasséli Coulibaly,
- Frederick Moore,
- Roy A. Hall,
- Paul R. Young,
- Jason M. Mackenzie,
- Jody Hobson-Peters,
- Andreas Suhrbier,
- Daniel Watterson,
- Alexander A. Khromykh
Affiliations
- Alberto A. Amarilla
- School of Chemistry and Molecular Biosciences, University of Queensland
- Julian D. J. Sng
- School of Chemistry and Molecular Biosciences, University of Queensland
- Rhys Parry
- School of Chemistry and Molecular Biosciences, University of Queensland
- Joshua M. Deerain
- Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne
- James R. Potter
- School of Chemistry and Molecular Biosciences, University of Queensland
- Yin Xiang Setoh
- School of Chemistry and Molecular Biosciences, University of Queensland
- Daniel J. Rawle
- QIMR Berghofer Medical Research Institute
- Thuy T. Le
- QIMR Berghofer Medical Research Institute
- Naphak Modhiran
- School of Chemistry and Molecular Biosciences, University of Queensland
- Xiaohui Wang
- School of Chemistry and Molecular Biosciences, University of Queensland
- Nias Y. G. Peng
- School of Chemistry and Molecular Biosciences, University of Queensland
- Francisco J. Torres
- School of Chemistry and Molecular Biosciences, University of Queensland
- Alyssa Pyke
- Queensland Health Forensic & Scientific Services, Queensland Department of Health
- Jessica J. Harrison
- School of Chemistry and Molecular Biosciences, University of Queensland
- Morgan E. Freney
- School of Chemistry and Molecular Biosciences, University of Queensland
- Benjamin Liang
- School of Chemistry and Molecular Biosciences, University of Queensland
- Christopher L. D. McMillan
- School of Chemistry and Molecular Biosciences, University of Queensland
- Stacey T. M. Cheung
- School of Chemistry and Molecular Biosciences, University of Queensland
- Darwin J. Da Costa Guevara
- School of Chemistry and Molecular Biosciences, University of Queensland
- Joshua M. Hardy
- Infection & Immunity Program, Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University
- Mark Bettington
- School of Medicine, University of Queensland
- David A. Muller
- School of Chemistry and Molecular Biosciences, University of Queensland
- Fasséli Coulibaly
- Infection & Immunity Program, Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University
- Frederick Moore
- Queensland Health Forensic & Scientific Services, Queensland Department of Health
- Roy A. Hall
- School of Chemistry and Molecular Biosciences, University of Queensland
- Paul R. Young
- School of Chemistry and Molecular Biosciences, University of Queensland
- Jason M. Mackenzie
- Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne
- Jody Hobson-Peters
- School of Chemistry and Molecular Biosciences, University of Queensland
- Andreas Suhrbier
- QIMR Berghofer Medical Research Institute
- Daniel Watterson
- School of Chemistry and Molecular Biosciences, University of Queensland
- Alexander A. Khromykh
- School of Chemistry and Molecular Biosciences, University of Queensland
- DOI
- https://doi.org/10.1038/s41467-021-23779-5
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 15
Abstract
Here the authors describe a simple reverse genetics method that relies on overlapping cDNA fragments for generation of positive-strand viruses including SARS-CoV-2 and characterize them in vitro and in vivo.
