Surface Properties and Biocompatibility of Anodized Titanium with a Potential Pretreatment for Biomedical Applications
Bai-Hung Huang,
Yi-Jung Lu,
Wen-Chien Lan,
Muhammad Ruslin,
Hung-Yang Lin,
Keng-Liang Ou,
Takashi Saito,
Hsin-Yu Tsai,
Chen-Han Lee,
Yung-Chieh Cho,
Tzu-Sen Yang,
Chung-Ming Liu,
Ping-Jen Hou
Affiliations
Bai-Hung Huang
Biomedical Technology R & D Center, China Medical University Hospital, Taichung 404, Taiwan
Yi-Jung Lu
Division of Family and Operative Dentistry, Taipei Medical University Hospital, Taipei 110, Taiwan
Wen-Chien Lan
Department of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung 203, Taiwan
Muhammad Ruslin
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Hasanuddin University, Makassar 90245, Indonesia
Hung-Yang Lin
Department of Dentistry, Fu Jen Catholic University Hospital, Fu Jen Catholic University, New Taipei City 242, Taiwan
Keng-Liang Ou
Biomedical Technology R & D Center, China Medical University Hospital, Taichung 404, Taiwan
Takashi Saito
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan
Hsin-Yu Tsai
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan
Chen-Han Lee
Taiwan Society of Blood Biomaterials, New Taipei City 221, Taiwan
Yung-Chieh Cho
Biomedical Technology R & D Center, China Medical University Hospital, Taichung 404, Taiwan
Tzu-Sen Yang
Graduate Institute of Biomedical Optomechatronics, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan
Chung-Ming Liu
Biomedical Technology R & D Center, China Medical University Hospital, Taichung 404, Taiwan
Ping-Jen Hou
Biomedical Technology R & D Center, China Medical University Hospital, Taichung 404, Taiwan
The effects of anodized titanium (Ti) with a potential hydrogen fluoride (HF) acid pretreatment through cathodization on the formation of nano-porous Ti dioxide (TiO2) layer were characterized using field-emission scanning electron microscopy, grazing incidence X-ray diffractometer, and contact angle goniometer. The biocompatibility was determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test. Analytical results found that a well-aligned nano-porous structure was formed on the anodized Ti surface with HF pretreatment concentration above 0.5%. Microstructure of the nano-porous Ti dioxide surface generated by anodization with HF pretreatment was composed of anatase and rutile phases, while the anodized Ti sample with HF pretreatment concentration of 0.5% presented excellent hydrophilicity surface. An in-vitro biocompatibility also indicated that osteoblast cells grown on the surface of the anodized Ti sample with HF pretreatment increased with the increase of culture time. The filopodia of osteoblast cells not only adhered flat, but also tightly grabbed the nano-porous structure for promoting cell adhesion and proliferation. Therefore, the anodized Ti with HF pretreatment can form a functionalized surface with great biocompatibility for biomedical applications, particularly for dental implants.
