Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation

Yunfei Zhang; Yali Liu; Wei Yan; Xuecong Zhang; Yu Hou; Zijing Zhou; Ruiyun Guo; Guobin Liu; Jun Ma

Stem Cell Research (Oct 2023)

Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation

Yunfei Zhang,
Yali Liu,
Wei Yan,
Xuecong Zhang,
Yu Hou,
Zijing Zhou,
Ruiyun Guo,
Guobin Liu,
Jun Ma

Affiliations

Yunfei Zhang: Emergency Department, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province 050000, China; Hebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Yali Liu: Child Health (Psychological Behavior) Department, Hebei Children's Hospital, Hebei Province 050017, China
Wei Yan: Hebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Xuecong Zhang: Hebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Yu Hou: Hebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Zijing Zhou: Hebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Ruiyun Guo: Hebei Medical University-University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Guobin Liu: The First Hospital of Hebei Medical University, Hebei Province 050000, China; Corresponding authors at: The First Hospital of Hebei Medical University, Hebei Province 050000, China (G. Liu); Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Medical University, Hebei Province 050017, China (J. Ma).
Jun Ma: Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Child Health (Psychological Behavior) Department, Hebei Children's Hospital, Hebei Province 050017, China; Corresponding authors at: The First Hospital of Hebei Medical University, Hebei Province 050000, China (G. Liu); Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Medical University, Hebei Province 050017, China (J. Ma).

Journal volume & issue: Vol. 72
p. 103201

Abstract

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In this study, fibroblasts were harvested and isolated from a healthy 14-year-old male donor and reprogrammed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc to generate human induced pluripotent stem cell (iPSC) lines. The resulting iPSCs were integration-free, expressed normal karyotype, displayed pluripotency markers, and have been demonstrated to differentiate into cells with three germ layer. And the iPSCs were further differentiated to chondrosphere in vivo. The models could be used to test multiple differentiation protocols and also as a control for screening drugs and studying cartilage related disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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