Concise overview of selected metal-based stains: Application in morphology

Abstract

Background: Modern research in the morphological sciences field requires various specialized research methods, including histological techniques. This concise review presents considerations on applying selected histochemical techniques in anatomical research. Aim: This article presents selected metal-based stains and their application in examining tissue morphology and structure using an optical microscope. Additionally, the article will explore the benefits, drawbacks, and widespread usage of these stains. Conclusions: Morphological research requires reliable observations. Thus, specific pros and cons of applying metallic stains must be evaluated. However, the use of metal-based stains in microanatomical and neuroanatomical research is still current.

Keywords

• Histological techniques
• Histochemistry
• Metal-based stains
• Tissue processing
• Tissue structure