Cytotoxic Effects of Lactobacilli Isolated from Azerbaijan Traditional Cheeses on Colorectal Tumor Cells HCT 116 and Identification of Paramount Strains

Journal of Ardabil University of Medical Sciences. 2018;18(2):191-203

 

Journal Homepage

Journal Title: Journal of Ardabil University of Medical Sciences

ISSN: 2228-7280 (Print); 2228-7299 (Online)

Publisher: Ardabil University of Medical Sciences

LCC Subject Category: Medicine: Medicine (General)

Country of publisher: Iran, Islamic Republic of

Language of fulltext: Persian

Full-text formats available: PDF, XML

 

AUTHORS

Mitra Rabiei ( Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran )
Gholamreza Zarrini ( Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran )
Majid Mahdavi ( Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran )

EDITORIAL INFORMATION

Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 6 weeks

 

Abstract | Full Text

Background & objectives: Colorectal cancer is one of the most common cancers in the world. Much attention has been given to nutritional supplements that can alter intestinal flora as factors preventing colon cancer. Research has shown that lactic acid bacteria in foods are potentially capable of inducing apoptosis. In this regard, the most focus has been on Lactobacillus genus. This study, investigated the cytotoxic effect of metabolites of isolated strain from Azerbaijan traditional cheeses on HCT116 colorectal cancer cells. Methods: In this cross-sectional study, after collecting samples of traditional "Lighvan" and "jug" cheeses in the region of Azerbaijan, MRS medium was used for isolation of lactobacilli. The isolates were identified by biochemical and molecular approaches after primary confirmation. The metabolites were produced in MRS broth, and their supernatants were separated. The inhibitory effect of the supernatants of the isolates on HCT116 cancer cells was studied and their effects were evaluated by microscopy and MTT assay. Results: In this study, three isolates of "Lighvan" and sixteen isolates of "jug" cheeses were obtained. The results of anticancer activity showed that the supernatants of the isolates CT2 and JT1 had a significant anticancer effect on HCT116 cancer cells (p˂0.05). Identification of the isolates CT2 and JT1 showed 99% and 96% similarity with Lactobacillus brevis, respectively. Conclusion: Lactobacilli in Azerbaijan traditional dairy products have a significant value in terms of anticancer properties.