Frontiers in Immunology (Mar 2021)
A Rapid and Efficient Screening System for Neutralizing Antibodies and Its Application for SARS-CoV-2
- Xiaojian Han,
- Xiaojian Han,
- Yingming Wang,
- Yingming Wang,
- Shenglong Li,
- Shenglong Li,
- Chao Hu,
- Chao Hu,
- Tingting Li,
- Tingting Li,
- Chenjian Gu,
- Kai Wang,
- Meiying Shen,
- Jianwei Wang,
- Jianwei Wang,
- Jie Hu,
- Ruixin Wu,
- Ruixin Wu,
- Song Mu,
- Song Mu,
- Fang Gong,
- Qian Chen,
- Qian Chen,
- Fengxia Gao,
- Fengxia Gao,
- Jingjing Huang,
- Jingjing Huang,
- Yingyi Long,
- Yingyi Long,
- Feiyang Luo,
- Feiyang Luo,
- Shuyi Song,
- Shuyi Song,
- Shunhua Long,
- Shunhua Long,
- Yanan Hao,
- Yanan Hao,
- Luo Li,
- Luo Li,
- Yang Wu,
- Wei Xu,
- Xia Cai,
- Qingzhu Gao,
- Guiji Zhang,
- Changlong He,
- Kun Deng,
- Li Du,
- Li Du,
- Yaru Nai,
- Yaru Nai,
- Wang Wang,
- Wang Wang,
- Youhua Xie,
- Di Qu,
- Ailong Huang,
- Ni Tang,
- Aishun Jin,
- Aishun Jin
Affiliations
- Xiaojian Han
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Xiaojian Han
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Yingming Wang
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Yingming Wang
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Shenglong Li
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Shenglong Li
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Chao Hu
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Chao Hu
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Tingting Li
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Tingting Li
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Chenjian Gu
- Key Laboratory of Medical Molecular Virology, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China
- Kai Wang
- Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China
- Meiying Shen
- Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, China
- Jianwei Wang
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Jianwei Wang
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Jie Hu
- Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China
- Ruixin Wu
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Ruixin Wu
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Song Mu
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Song Mu
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Fang Gong
- Department of Pediatrics, Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China
- Qian Chen
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Qian Chen
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Fengxia Gao
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Fengxia Gao
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Jingjing Huang
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Jingjing Huang
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Yingyi Long
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Yingyi Long
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Feiyang Luo
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Feiyang Luo
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Shuyi Song
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Shuyi Song
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Shunhua Long
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Shunhua Long
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Yanan Hao
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Yanan Hao
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Luo Li
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Luo Li
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Yang Wu
- Key Laboratory of Medical Molecular Virology, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China
- Wei Xu
- Key Laboratory of Medical Molecular Virology, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China
- Xia Cai
- Key Laboratory of Medical Molecular Virology, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China
- Qingzhu Gao
- Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China
- Guiji Zhang
- Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China
- Changlong He
- Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China
- Kun Deng
- Department of Laboratory, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, China
- Li Du
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Li Du
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Yaru Nai
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Yaru Nai
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Wang Wang
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Wang Wang
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- Youhua Xie
- Key Laboratory of Medical Molecular Virology, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China
- Di Qu
- Key Laboratory of Medical Molecular Virology, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China
- Ailong Huang
- Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China
- Ni Tang
- Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China
- Aishun Jin
- Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China
- Aishun Jin
- Chongqing Key Laboratory of Basic and Translational Research of Tumor Immunology, Chongqing Medical University, Chongqing, China
- DOI
- https://doi.org/10.3389/fimmu.2021.653189
- Journal volume & issue
-
Vol. 12
Abstract
After the pandemic of COVID-19, neutralizing antibodies (NAbs) against SARS-CoV-2 have been developed for the prophylactic and therapeutic purposes. However, few methodologies are described in detail on how to rapidly and efficiently generate effective NAbs to SARS-CoV-2. Here, we integrated and optimized a strategically screening method for NAbs, which has enabled us to obtain SARS-CoV-2 receptor-binding domain (RBD) specific NAbs within 6 days, followed by additional 9 days for antibody production and function analysis. Using this method, we obtained 198 specific Abs against SARS-CoV-2 RBD from the blood samples of COVID-19 convalescent patients, and 96 of them showed neutralizing activity. At least 20% of these NAbs exhibited advanced neutralizing potency and high affinity, with the top two NAbs showing half-maximal inhibitory concentration (IC50) to block authentic SARS-CoV-2 at 9.88 and 11.13 ng/ml, respectively. Altogether, our study provides an effective methodology with high applicable value for discovering potential preventative and therapeutic NAbs for the emerging infectious diseases.
Keywords
