New Insights into Bioactive Compounds from the Medicinal Plant <i>Spathodea campanulata</i> P. Beauv. and Their Activity against <i>Helicobacter pylori</i>
Corinne Raïssa Ngnameko,
Lucia Marchetti,
Barbara Zambelli,
Antonio Quotadamo,
Davide Roncarati,
Davide Bertelli,
Frederic Nico Njayou,
Stella I. Smith,
Paul F. Moundipa,
Maria Paola Costi,
Federica Pellati
Affiliations
Corinne Raïssa Ngnameko
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
Lucia Marchetti
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
Barbara Zambelli
Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna, Viale Fanin 44, 40127 Bologna, Italy
Antonio Quotadamo
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
Davide Roncarati
Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna, Viale Fanin 44, 40127 Bologna, Italy
Davide Bertelli
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
Frederic Nico Njayou
Department of Biochemistry, Faculty of Science, The University of Yaounde I, P. Box 812 Yaounde, Cameroon
Stella I. Smith
Nigerian Institute of Medical Research, PMB 2013, Yaba, Lagos 100001, Nigeria
Paul F. Moundipa
Department of Biochemistry, Faculty of Science, The University of Yaounde I, P. Box 812 Yaounde, Cameroon
Maria Paola Costi
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
Federica Pellati
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
The medicinal plant Spathodea campanulata P. Beauv. (Bignoniaceae) has been traditionally applied for the prevention and treatment of diseases of the kidney and urinary system, the skin, the gastrointestinal tract, and inflammation in general. The present work shows for the first time how chemical components from this plant inhibit Helicobacter pylori growth by urease inhibition and modulation of virulence factors. The crude extract and the main fractions of S. campanulata bark were tested on H. pylori isolated strains and the active ones were further fractionated. Fractions and sub-fractions of the plant crude extract were characterized by ultra-high-performance liquid chromatographic tandem high resolution-mass spectrometry detection (UHPLC-HRMS). Several phenolics and triterpenoids were identified. Among the sub-fractions obtained, SB2 showed the capacity to inhibit H. pylori urease in a heterologous bacterial model. One additional sub-fraction (SE3) was able to simultaneously modulate the expression of two adhesins (HopZ and BabA) and one cytotoxin (CagA). The flavonol kaempferol was identified as the most interesting compound that deserves further investigation as a new hit for its capacity to modulate H. pylori virulence factors.
