Crops (Apr 2024)

A Genome-Wide Association Study of Seed Morphology-Related Traits in Sorghum Mini-Core and Senegalese Lines

  • Ezekiel Ahn,
  • Sunchung Park,
  • Zhenbin Hu,
  • Vishnutej Ellur,
  • Minhyeok Cha,
  • Yoonjung Lee,
  • Louis K. Prom,
  • Clint Magill

DOI
https://doi.org/10.3390/crops4020012
Journal volume & issue
Vol. 4, no. 2
pp. 156 – 171

Abstract

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Sorghum (Sorghum bicolor L.) ranks fifth as the most crucial cereal crop globally, yet its seed morphology remains relatively unexplored. This study investigated seed morphology in sorghum based on 115 mini-core and 130 Senegalese germplasms. Eight seed morphology traits encompassing size, shape, and color parameters were assessed. Statistical analyses explored potential associations between these traits and resistance to three major sorghum diseases: anthracnose, head smut, and downy mildew. Furthermore, genome-wide association studies (GWAS) were conducted using phenotypic data from over 24,000 seeds and over 290,000 publicly available single nucleotide polymorphisms (SNPs) through the Genome Association and Prediction Integrated Tool (GAPIT) R package. Significant SNPs associated with various seed morphology traits were identified and mapped onto the reference sorghum genome to identify novel candidate defense genes.

Keywords