Scientific Reports (Aug 2024)
The effect of exogenous gibberellin and its synthesis inhibitor treatments for morphological and physiological characteristics of Tartary buckwheat
Abstract
Abstract Gibberellin (GA3) is an important plant hormone involved in many physiological and developmental processes in plants. However, the physiological mechanism of GA3 on the regulation yield and grain shell thickness of Tartary buckwheat is still unclear. In this study, the thick-shelled cultivar “Jinqiao 2” and thin-shelled cultivar “Miku 18” were used to study the effects of different concentrations (0, 50, and 100 mg L−1) of exogenous GA3 and chlorocholine chloride (CCC, GA3 synthesis inhibitor) on the cellulose content, amylase, and sucrose synthase (SS) activity in grain shell and the yield of Tartary buckwheat. The application of exogenous GA3 can improve the cellulose content and the activity of amylase and SS in the grain shell of the two Tartary buckwheat varieties. It can also increase the main stem node number, main stem branch number, grains per plant, and yield. Compared with the control treatment (CK, 0 mg L−1), the 100 mg/L exogenous GA3 treatment increased the number of grains per plant, grain weight per plant, 1000-grain weight, and yield of Jinqiao 2 by 20.1%, 41.9%, 13%, and 34.7%, respectively. These items of Miku 18 were increased by 26%, 15.2%, 10.2%, and 23.8%. The application of CCC reduced the activity of amylase and SS and cellulose content in grain shell. In addition, it decreased the main stem node number, main stem branch number, grains per plant, and yield of Tartary buckwheat. In summary, exogenous GA3 treatment not only improved the yield of Tartary buckwheat but also increased the thickness of grain shell by enhancing the activity of amylase and SS and promoting the synthesis and accumulation of cellulose. The results can provide theoretical references for clarifying the physiological mechanism of the difference in shell thickness between Tartary buckwheat varieties.
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