Cloning and bioinformatic analysis of PintGOBP2 gene in Plodiainterpunctella (Hübener)

Abstract

The Indian meal moth, Plodiainterpunctella (Hübner) (Lepidoptera: Pyralidae, Pyralidae) is a kind of destructive polyphagous storage pests that is widely distributed in China. The odorant binding protein (OBPs) plays an important role in the behavioral response of the Indian moth in infection of its host. An Indian meal mothOBP gene sequence was obtained by screening and identifying the transcriptome dataand named PintGOBP2, which was analyzed by DNAMAN and homology comparison was performed by BLAST, phylogenetic trees were constructed using MEGA 6.0. The results showed that the open reading frame of the PintGOBP2 was 426 bp, encoding 141 amino acids with a molecular weight 15.48 kDa, and an isoelectric point of 4.39; the deduced amino acid sequence possesses a putative signal peptide of 16 amino acid residues at the N terminus. There are 6 conservative cysteine sites in the protein sequence, belongs to the Classical OBPs family genes. Homologous alignment showed that PintGOBP2 has high similarity with other lepidoptera insects GOBP. Further Phylogenetic analysis showed that PintGOBP2 could be clustered in the GOBP superfamily of lepidoptera insects. The results provide a molecular basis for further analysis of the physiological function of PintGOBP2 in the process of olfactory signal transmission in the P. interpunctella.

Keywords

• plodiainterpunctella (hübner)
• general odorant binding protein
• gene cloning
• bioinformatics analysis