Ultra‐high static magnetic fields cause immunosuppression through disrupting B‐cell peripheral differentiation and negatively regulating BCR signaling
Heng Gu,
Yufan Fu,
Biao Yu,
Li Luo,
Danqing Kang,
Miaomiao Xie,
Yukai Jing,
Qiuyue Chen,
Xin Zhang,
Juan Lai,
Fei Guan,
Huamei Forsman,
Junming Shi,
Lu Yang,
Jiahui Lei,
Xingrong Du,
Xin Zhang,
Chaohong Liu
Affiliations
Heng Gu
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Yufan Fu
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Biao Yu
High Magnetic Field Laboratory Hefei Institutes of Physical Science Chinese Academy of Sciences Hefei Anhui China
Li Luo
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Danqing Kang
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Miaomiao Xie
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Yukai Jing
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Qiuyue Chen
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Xin Zhang
GeneMind Biosciences Company Limited Shenzhen China
Juan Lai
GeneMind Biosciences Company Limited Shenzhen China
Fei Guan
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Huamei Forsman
Department of Rheumatology and Inflammation Research Institute of Medicine Sahlgrenska Academy University of Gothenburg Goteborg Sweden
Junming Shi
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Lu Yang
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Jiahui Lei
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Xingrong Du
Shanghai Key Laboratory of Metabolic Remodeling and Health Institute of Metabolism and Integrative Biology Fudan University Shanghai China
Xin Zhang
High Magnetic Field Laboratory Hefei Institutes of Physical Science Chinese Academy of Sciences Hefei Anhui China
Chaohong Liu
Department of Pathogen Biology School of Basic Medicine Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonostic Infectious Disease Huazhong University of Science and Technology Wuhan China
Abstract To increase the imaging resolution and detection capability, the field strength of static magnetic fields (SMFs) in magnetic resonance imaging (MRI) has significantly increased in the past few decades. However, research on the side effects of high magnetic field is still very inadequate and the effects of SMF above 1 T (Tesla) on B cells have never been reported. Here, we show that 33.0 T ultra‐high SMF exposure causes immunosuppression and disrupts B cell differentiation and signaling. 33.0 T SMF treatment resulted in disturbance of B cell peripheral differentiation and antibody secretion and reduced the expression of IgM on B cell membrane, and these might be intensity dependent. In addition, mice exposed to 33.0 T SMF showed inhibition on early activation of B cells, including B cell spreading, B cell receptor clustering and signalosome recruitment, and depression of both positive and negative molecules in the proximal BCR signaling, as well as impaired actin reorganization. Sequencing and gene enrichment analysis showed that SMF stimulation also affects splenic B cells' transcriptome and metabolic pathways. Therefore, in the clinical application of MRI, we should consider the influence of SMF on the immune system and choose the optimal intensity for treatment.
