Obtaining and characterization of monoclonal antibodies against capsular polysaccharides of Streptococcus pneumoniae serotype 1, 5, 6B, 14 and 19F

Abstract

Five monoclonal antibodies named 2B5B10, 2E4D11, 1D12E10, 1D1D11 and 177/10/27/11 were produced against capsular polysaccharides of Streptococcus pneumoniae serotypes 1, 5, 6B, 14 and 19F respectively. They all were obtained by hybridoma technology and produced by in vivo procedures. Were purified by Protein A-based Affinity Chromatography and the yield of all processes was around the 2.9 and 3.7 mg per mL of ascitic fluid; and with a high purity (over 90% in all cases). All MAbs only reacted against their homologue polysaccharide showing no cross reactivity against other serogroups. The affinity constants (Kaff) measured by non-competitive ELISA were 3.08 x 1011 M-1 for 2B5B10, 1.53 x 1012 M-1 for 2E4D11, 8.68 x 1010 M-1 for 1D12E10, 1.42 x 1013 M-1 for 1D1D11 and 7.2 109 M-1 for 177/10/27/11. The potential application of these MAbs for identity tests was demonstrated by their abilities of no cross-react with the other purified PS presents in QuimiVio, through indirect ELISA

Keywords

• monoclonal antibodies
• Streptococcus pneumoniae
• identity tests
• ELISA