The ameliorative effects of a phenolic derivative of Moringa oleifera leave against vanadium-induced neurotoxicity in mice
Olumayowa O. Igado,
Anna Andrioli,
Idris A. Azeez,
Francesco Girolamo,
Mariella Errede,
Oluwasanmi O. Aina,
Jan Glaser,
Ulrike Holzgrabe,
Marina Bentivoglio,
James O. Olopade
Affiliations
Olumayowa O. Igado
Department of Veterinary Anatomy, University of Ibadan, Ibadan, Nigeria; Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Italy; Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari School of Medicine, Bari, Italy
Anna Andrioli
Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Italy
Idris A. Azeez
Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Italy
Francesco Girolamo
Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari School of Medicine, Bari, Italy
Mariella Errede
Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari School of Medicine, Bari, Italy
Oluwasanmi O. Aina
Department of Veterinary Anatomy, University of Ibadan, Ibadan, Nigeria
Jan Glaser
Institut of Pharmacy and Food Chemistry, University of Würzburg, Germany
Ulrike Holzgrabe
Institut of Pharmacy and Food Chemistry, University of Würzburg, Germany
Marina Bentivoglio
Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Italy
James O. Olopade
Department of Veterinary Anatomy, University of Ibadan, Ibadan, Nigeria; Corresponding author at: Department of Veterinary Anatomy, University of Ibadan, Nigeria.
Vanadium, a transition series metal released during some industrial activities, induces oxidative stress and lipid peroxidation. Ameliorative effect of a pure compound from the methanolic extract of Moringa oleifera leaves, code-named MIMO2, in 14-day old mice administered with vanadium (as sodium metavanadate 3 mg/kg) for 2 weeks was assessed. Results from body weight monitoring, muscular strength, and open field showed slight reduction in body weight and locomotion deficit in vanadium-exposed mice, ameliorated with MIMO2 co-administration. Degeneration of the Purkinje cell layer and neuronal death in the hippocampal CA1 region were observed in vanadium-exposed mice and both appeared significantly reduced with MIMO2 co-administration. Demyelination involving the midline of the corpus callosum, somatosensory and retrosplenial cortices was also reduced with MIMO2. Microglia activation and astrogliosis observed through immunohistochemistry were also alleviated. Immunohistochemistry for myelin, axons and oligodendrocyte lineage cells were also carried out and showed that in vanadium-treated mice brains, oligodendrocyte progenitor cells increased NG2 immunolabelling with hypertrophy and bushy, ramified appearance of their processes. MIMO2 displayed ameliorative and antioxidative effects in vanadium-induced neurotoxicity in experimental murine species. This is likely the first time MIMO2 is being used in vivo in an animal model.
