Effect of Electrospinning Process on Total Antioxidant Activity of Electrospun Nanofibers Containing Grape Seed Extract

Abstract

Electrospinning is a common technique used for the production of nanofibers, and it is based on the fact that the electrically charged liquid polymer is positioned in a continuous fiber form on a grounded surface. Grape seed is rich in phenolic compounds and can be used as a dietary supplement or as a natural antioxidant source in diet. In this study, grape seed extract of Burdur Dimrit variety (Vitis vinifera L.) was electrospun with gelatin, polyvinyl alcohol (PVA) and PVA/β-cyclodextrin polymers to produce nanofibers with antioxidant activity. The aim of this study was to determine the effect of the electrospinning process on the total antioxidant activity and total phenolic contents of electrospun polymers with grape seed extracts. Total antioxidant activity of samples (by ABTS and DPPH assays) and total phenolic contents (Folin–Ciocalteu method) were determined before and after the electrospinning process of polymers with grape seed extract. Electrospinning with gelatin polymer decreased the antioxidant activity (ABTS assay) of nanofibers containing grape seed extract by 65% and their total phenolic contents by 7%. However, electrospinning treatment with PVA and PVA/β-cyclodextrin had no effect on the total antioxidant activity (ABTS and DPPH) and total phenolic substance contents of grape seed extract nanofibers.

Keywords

• electrospinning
• polyvinyl alcohol
• grape seed
• antioxidant
• gelatin