Oxygen tension modulates the mitochondrial genetic bottleneck and influences the segregation of a heteroplasmic mtDNA variant in vitro

Mikael G. Pezet; Aurora Gomez-Duran; Florian Klimm; Juvid Aryaman; Stephen Burr; Wei Wei; Mitinori Saitou; Julien Prudent; Patrick F. Chinnery

doi:10.1038/s42003-021-02069-2

Communications Biology (May 2021)

Oxygen tension modulates the mitochondrial genetic bottleneck and influences the segregation of a heteroplasmic mtDNA variant in vitro

Mikael G. Pezet,
Aurora Gomez-Duran,
Florian Klimm,
Juvid Aryaman,
Stephen Burr,
Wei Wei,
Mitinori Saitou,
Julien Prudent,
Patrick F. Chinnery

Affiliations

Mikael G. Pezet: Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus
Aurora Gomez-Duran: Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus
Florian Klimm: Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus
Juvid Aryaman: Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus
Stephen Burr: Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus
Wei Wei: Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus
Mitinori Saitou: Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University
Julien Prudent: Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus
Patrick F. Chinnery: Department of Clinical Neurosciences, School of Clinical Medicine, University of Cambridge, Cambridge Biomedical Campus

DOI: https://doi.org/10.1038/s42003-021-02069-2
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 12

Abstract

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Using an in vitro culture system, Pezet et al. studied the influence of oxygen on the mitochondrial DNA (mtDNA) in primordial germ cell-like cells (PGCLCs) in vitro. Low oxygen levels resembling in vivo reduced the cell mtDNA content causing a genetic bottleneck and the segregation of different mtDNA genotypes.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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