RUDN Journal of Medicine (Dec 2013)

Evaluation of effectiveness of solvent-detergent viral in activation in the manufacture of intravenous human immunoglobulin G in a model of virus diarrhea — mucosal disease of cattle

  • A V Kaz’yanin,
  • N V Zubkova,
  • I V Krasilnikov,
  • A K Lobastova,
  • V V Anastasiev,
  • A M Nikolaeva,
  • T I Glotova,
  • O V Kungurtseva,
  • A G Glotov

Journal volume & issue
Vol. 0, no. 4
pp. 24 – 30

Abstract

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In model experiments using in vitro virus diarrhea — mucosal disease in cattle (bovine VD-MDC) studied the effectiveness of solvent-detergent (SD) virus inactivation used in the production of intravenous human immunoglobulin G (IgG). Optimized conditions for SD processing of immunoglobulin: the final concentration of tributylphosphate (TBP) 0.3% 0.2% sodium cholate, process temperature of 30 ± 1 °C to 36 ± 1 °C, incubation time 6 hours. The level of reduction of VD-MDC in the processing of the drug in these conditions was more than 6 log 10 TCD 50/ml.

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