Polymorphism of microsatellite loci in bread wheat (Triticum aestivum L.) and related species

Abstract

This study analysed polymorphism of 15 microsatellite loci in the col­lection comprising of 40 genotypes of bread wheat (Triticum aestivum L.), 32 genotypes belonging to other species within Triticum genus and 3 genotypes from Aegilops genus. The results showed significant differences in the variability of the tested loci in bread wheat and related species. In the collection of bread wheat genotypes, 119 alleles were detected with the average number of 7.9 alleles per locus. In wild and cultivated related species 157 alleles were identified, with the average of 10.5 alleles per locus. All analysed parameters of micro­satellite loci variability (PIC value, gene diversity, heterozygosity, etc.) indicated higher level of polymorphism in wild relatives than in the cultivated bread wheat. Analyses of individual genomes indicated that in the bread wheat genetic diversity of the B and D genomes was significantly reduced in relation to the A genome, while the differences in polymorphism between genomes in the wild relatives were significantly lower. The results showed that wild related species can be used as sources for new variability in wheat breeding. [Project of the Serbian Ministry of Education, Science and Technological Development]

Keywords

• Aegilops ssp.
• Triticum sp.
• SSR markers
• locus variability