Simultaneous recordings of pupil size variation and locus coeruleus activity in mice

Marine Megemont; Lucas S. Tortorelli; Jim McBurney-Lin; Jeremiah Y. Cohen; Daniel H. O’Connor; Hongdian Yang

STAR Protocols (Mar 2024)

Simultaneous recordings of pupil size variation and locus coeruleus activity in mice

Marine Megemont,
Lucas S. Tortorelli,
Jim McBurney-Lin,
Jeremiah Y. Cohen,
Daniel H. O’Connor,
Hongdian Yang

Affiliations

Marine Megemont: Department of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA 92521, USA; Corresponding author
Lucas S. Tortorelli: Department of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA 92521, USA
Jim McBurney-Lin: Department of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA 92521, USA; Neuroscience Graduate Program, University of California, Riverside, Riverside, CA 92521, USA
Jeremiah Y. Cohen: Solomon H. Snyder Department of Neuroscience & Krieger Mind/Brain Institute, Johns Hopkins University, Baltimore, MD 21218, USA
Daniel H. O’Connor: Solomon H. Snyder Department of Neuroscience & Krieger Mind/Brain Institute, Johns Hopkins University, Baltimore, MD 21218, USA
Hongdian Yang: Department of Molecular, Cell and Systems Biology, University of California, Riverside, Riverside, CA 92521, USA; Neuroscience Graduate Program, University of California, Riverside, Riverside, CA 92521, USA; Corresponding author

Journal volume & issue: Vol. 5, no. 1
p. 102785

Abstract

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Summary: An extensive literature describes how pupil size reflects neuromodulatory activity, including the noradrenergic system. Here, we present a protocol for the simultaneous recording of optogenetically identified locus coeruleus (LC) units and pupil diameter in mice under different conditions. We describe steps for building an optrode, performing surgery to implant the optrode and headpost, searching for opto-tagged LC units, and performing dual LC-pupil recording. We then detail procedures for data processing and analysis.For complete details on the use and execution of this protocol, please refer to Megemont et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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