Smc5/6’s multifaceted DNA binding capacities stabilize branched DNA structures

Jeremy T-H. Chang; Shibai Li; Emily C. Beckwitt; Thane Than; Cory Haluska; Joshua Chandanani; Michael E. O’Donnell; Xiaolan Zhao; Shixin Liu

doi:10.1038/s41467-022-34928-9

Nature Communications (Nov 2022)

Smc5/6’s multifaceted DNA binding capacities stabilize branched DNA structures

Jeremy T-H. Chang,
Shibai Li,
Emily C. Beckwitt,
Thane Than,
Cory Haluska,
Joshua Chandanani,
Michael E. O’Donnell,
Xiaolan Zhao,
Shixin Liu

Affiliations

Jeremy T-H. Chang: Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University
Shibai Li: Molecular Biology Program, Memorial Sloan Kettering Cancer Center
Emily C. Beckwitt: Laboratory of DNA Replication, Howard Hughes Medical Institute, The Rockefeller University
Thane Than: Molecular Biology Program, Memorial Sloan Kettering Cancer Center
Cory Haluska: Molecular Biology Program, Memorial Sloan Kettering Cancer Center
Joshua Chandanani: Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University
Michael E. O’Donnell: Laboratory of DNA Replication, Howard Hughes Medical Institute, The Rockefeller University
Xiaolan Zhao: Tri-Institutional MD-PhD Program, The Rockefeller University, Weill Cornell Medical College, and Memorial Sloan Kettering Cancer Center
Shixin Liu: Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University

DOI: https://doi.org/10.1038/s41467-022-34928-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

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Using single-molecule visualization and manipulation, Chang et al. show that the eukaryotic Smc5/6 complex preferentially binds to and stabilizes ssDNA-dsDNA junctions, which could serve as the molecular basis for its diverse roles in genome maintenance.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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