Molecular detection of two virulence factors of Pseudomonas aeruginosa isolated from burn patients

Abstract

The present study aimed to identify two virulence genes in P. aeruginosa isolated from infected burn wound. Out of 70 burn swabs, 54 (77%) showed positive growth on enriched media which obtained from burn wound at Al-Mussan Teaching Hospital in Mussan province, Iraq. Only 13 isolates (35%) identified as P. aeruginosa, and those isolates examined using Polymerase Chain Reaction (PCR) for detection the exoS and phzS genes. The results recorded that 46% and 77% of isolates have exoS, phzS genes, respectively. We concluded a P. aeruginosa harbor diverse percentages of both genes as virulence factors, and those factors must be increased the pathogenicity of this pathogen.

Keywords

• P. aeruginosa
• burn
• exoS
• phzS genes
• virulence factors