Endocrine Regulations (Jul 2020)

Expression of IDE and PITRM1 genes in ERN1 knockdown U87 glioma cells: effect of hypoxia and glucose deprivation

  • Minchenko Dmytro O.,
  • Khita Olena O.,
  • Tsymbal Dariia O.,
  • Danilovskyi Serhij V.,
  • Rudnytska Olha V.,
  • Halkin Oleh V.,
  • Kryvdiuk Iryna V.,
  • Smeshkova Maria V.,
  • Yakymchuk Mykhailo M.,
  • Bezrodnyi Borys H.,
  • Minchenko Oleksandr H.

DOI
https://doi.org/10.2478/enr-2020-0021
Journal volume & issue
Vol. 54, no. 3
pp. 183 – 195

Abstract

Read online

Objective. The aim of the present investigation was to study the expression of genes encoding polyfunctional proteins insulinase (insulin degrading enzyme, IDE) and pitrilysin metallopeptidase 1 (PITRM1) in U87 glioma cells in response to inhibition of endoplasmic reticulum stress signaling mediated by ERN1/IRE1 (endoplasmic reticulum to nucleus signaling 1) for evaluation of their possible significance in the control of metabolism through ERN1 signaling as well as hypoxia, glucose and glutamine deprivations.

Keywords