Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia

Lifang Zou; Lifang Zou; Xinyao Han; Shuangmei Liu; Shuangmei Liu; Yingxin Gong; Bing Wu; Bing Wu; Zhihua Yi; Zhihua Yi; Hui Liu; Hui Liu; Shanhong Zhao; Shanhong Zhao; Tianyu Jia; Tianyu Jia; Lin Li; Lin Li; Huilong Yuan; Huilong Yuan; Liran Shi; Liran Shi; Chunping Zhang; Chunping Zhang; Yun Gao; Yun Gao; Guilin Li; Guilin Li; Hong Xu; Hong Xu; Shangdong Liang; Shangdong Liang

doi:10.3389/fphys.2018.00928

Frontiers in Physiology (Jul 2018)

Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia

Lifang Zou,
Lifang Zou,
Xinyao Han,
Shuangmei Liu,
Shuangmei Liu,
Yingxin Gong,
Bing Wu,
Bing Wu,
Zhihua Yi,
Zhihua Yi,
Hui Liu,
Hui Liu,
Shanhong Zhao,
Shanhong Zhao,
Tianyu Jia,
Tianyu Jia,
Lin Li,
Lin Li,
Huilong Yuan,
Huilong Yuan,
Liran Shi,
Liran Shi,
Chunping Zhang,
Chunping Zhang,
Yun Gao,
Yun Gao,
Guilin Li,
Guilin Li,
Hong Xu,
Hong Xu,
Shangdong Liang,
Shangdong Liang

Affiliations

Lifang Zou: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Lifang Zou: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Xinyao Han: First Clinical Department, Medical School of Nanchang University, Nanchang, China
Shuangmei Liu: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Shuangmei Liu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Yingxin Gong: First Clinical Department, Medical School of Nanchang University, Nanchang, China
Bing Wu: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Bing Wu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Zhihua Yi: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Zhihua Yi: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Hui Liu: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Hui Liu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Shanhong Zhao: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Shanhong Zhao: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Tianyu Jia: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Tianyu Jia: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Lin Li: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Lin Li: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Huilong Yuan: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Huilong Yuan: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Liran Shi: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Liran Shi: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Chunping Zhang: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Chunping Zhang: Department of Cell Biology, Medical School of Nanchang University, Nanchang, China
Yun Gao: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Yun Gao: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Guilin Li: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Guilin Li: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Hong Xu: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Hong Xu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Shangdong Liang: Department of Physiology, Medical School of Nanchang University, Nanchang, China
Shangdong Liang: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China

DOI: https://doi.org/10.3389/fphys.2018.00928
Journal volume & issue: Vol. 9

Abstract

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Myocardial ischemia (MI) is one of the major causes of death in cardiac diseases. Purinergic signaling is involved in bidirectional neuronal-glial communication in the primary sensory ganglia. The sensory neuritis of cardiac afferent neurons in cervical dorsal root ganglion (cDRG) interacts with cardiac sympathetic efferent postganglionic neurons, forming feedback loops. The P2Y12 receptor is expressed in satellite glial cells (SGCs) of DRG. Baicalin is a major active ingredient extracted from natural herbal medicines, which has anti-inflammatory and strong anti-oxidation properties. In this study we investigated the effect of baicalin on P2Y12 receptor in the cervical DRG SGC-mediated sympathoexcitatory reflex, which is increased during MI. The results showed that the expression of P2Y12 receptor mRNA and protein in DRG, and the co-localization values of P2Y12 receptor and glial fibrillary acidic protein (GFAP) in cDRG SGCs were increased after MI. The activated SGCs increased IL-1β protein expression and elevated Akt phosphorylation in cDRG. Baicalin treatment inhibited the upregulation of the P2Y12 receptor, GFAP protein and Akt phosphorylation in cDRG neurons/SGCs. The stellate ganglia (SG) affect cardiac sympathetic activity. Baicalin treatment also decreased the upregulation of the P2Y12 receptor, GFAP protein in the SG. The P2Y12 agonist, 2Me-SADP, increased [Ca2+]i in HEK293 cells transfected with the P2Y12 receptor plasmid and SGCs in cDRG. These results indicate that application of baicalin alleviates pathologic sympathetic activity induced by MI via inhibition of afferents in the cDRG.

Published in Frontiers in Physiology

ISSN: 1664-042X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Physiology
Website: https://www.frontiersin.org/journals/physiology

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