Cancer Management and Research (Dec 2021)
Prognosis Value of microRNA-3677-3p in Lung Adenocarcinoma and Its Regulatory Effect on Tumor Progression
Abstract
Jian Zhao,1,* Hanbing Yu,1,* Tianci Han,1,* Xiangyu Zhu2 1Third Department of Thoracic Surgery, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning, People’s Republic of China; 2Department of General Medicine, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiangyu ZhuDepartment of General Medicine, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning, People’s Republic of ChinaTel/Fax +86-24-31916684Email [email protected]: Lung adenocarcinomas (LUAD) was the most common subtype of lung cancer, and may result in a poor prognosis. This study was designed to explore the role of miR-3677-3p in LUAD and discuss in what way it functions in LUAD.Materials and Methods: We used RT-qPCR method to detect the expression levels of miR-3677-3p in 105 pairs of LUAD tissues and noncancerous tissues, as also as in LUAD cells. We used χ2 test to analyze the correlation between miR-3677-3p level and the clinical data. The prognosis significance of miR-3677-3p was inferred with Kaplan–Meier and multivariate Cox regression assays. Biological functions of LUAD cells were accessed by cell counting kit-8, transwell migration and invasion assay. The target gene of miR-3677-3p was investigated by luciferase activity assay.Results: miR-3677-3p represented an ascendant expression in LUAD tissue specimens and cells. miR-3677-3p expression was associated with the TNM stage and with solitary metastasis. Over-expression of miR-3677-3p can shorten the overall survival period of LUAD patients when compared with low expression. Knockdown of miR-3677-3p suppressed the biology function of NSCLC cells including proliferation, migration, and invasion. KLF12 was a target gene of miR-3677-3p.Conclusion: miR-3677-3p represents as a potential prognostic biomarker for LUAD. miR-3677-3p can promote LUAD progression by targeting KLF12.Keywords: miR-3677-3p, lung adenocarcinomas, prognosis, KLF12
