Frontiers in Physiology (Apr 2019)
A Novel Regulator of Preadipocyte Differentiation, Transcription Factor TCF21, Functions Partially Through Promoting LPL Expression
- Xinyang Zhang,
- Xinyang Zhang,
- Xinyang Zhang,
- Bohan Cheng,
- Bohan Cheng,
- Bohan Cheng,
- Chang Liu,
- Chang Liu,
- Chang Liu,
- Zhiqiang Du,
- Zhiqiang Du,
- Zhiqiang Du,
- Hui Zhang,
- Hui Zhang,
- Hui Zhang,
- Ning Wang,
- Ning Wang,
- Ning Wang,
- Mengqi Wu,
- Mengqi Wu,
- Mengqi Wu,
- Yumao Li,
- Yumao Li,
- Yumao Li,
- Zhiping Cao,
- Zhiping Cao,
- Zhiping Cao,
- Hui Li,
- Hui Li,
- Hui Li
Affiliations
- Xinyang Zhang
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Xinyang Zhang
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Xinyang Zhang
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- Bohan Cheng
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Bohan Cheng
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Bohan Cheng
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- Chang Liu
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Chang Liu
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Chang Liu
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- Zhiqiang Du
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Zhiqiang Du
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Zhiqiang Du
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- Hui Zhang
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Hui Zhang
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Hui Zhang
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- Ning Wang
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Ning Wang
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Ning Wang
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- Mengqi Wu
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Mengqi Wu
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Mengqi Wu
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- Yumao Li
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Yumao Li
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Yumao Li
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- Zhiping Cao
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Zhiping Cao
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Zhiping Cao
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- Hui Li
- Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China
- Hui Li
- Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China
- Hui Li
- College of Animal Science and Technology, Northeast Agricultural University, Harbin, China
- DOI
- https://doi.org/10.3389/fphys.2019.00458
- Journal volume & issue
-
Vol. 10
Abstract
The transcription factor TCF21 has been previously shown to be specifically expressed in white preadipocytes in mice. However, the exact biological function of TCF21 in the context of adipogenesis remains unknown. In the current study, we used chicken lines selected based on their abdominal fat content, and observed a significant decrease in TCF21 mRNA and protein levels in the abdominal fat of lean broilers relative to fat broilers. Moreover, TCF21 expression increased throughout preadipocyte differentiation in vitro. We also found that TCF21 knockdown and over-expression attenuated and promoted preadipocyte differentiation, respectively, as evidenced by appropriate changes in lipid droplet accumulation and altered expressions of C/EBPa, LPL, and A-FABP. Additional chromatin immunoprecipitation analyses and luciferase assays demonstrated that TCF21 promotes the transcription of LPL by directly binding to the E-box motif in the LPL promoter. Together, these results show that TCF21 is a novel regulator of preadipocyte differentiation, in part by directly promoting LPL expression.
Keywords
