Role of Root Exudates in Adaptative Reactions of Buckwheat Plants in Aluminium-acid Stress

Abstract

Aliminium toxicity is major limiting factor of crop production in acidic soils. It is known that mechanisms of toxic effects of aluminium are differing in biochemical characters, research of aluminium toxicity complicated by variety of its chemical forms and migration in soil and water ability. The root exudates qualitative composition of common buckwheat was evaluated. Organic complexing agents – oxalic acid and phenolic compounds were revealed. The role of these complexing agents in the buckwheat aluminium resistance under acidic stress, participation in processes of external and internal detoxification was shown. Spectrophometric assay revealed an increase in root secretion of oxalic acid by 2.5 times and decrease in content of phenolic compounds in root exudates solution by 3 times upon aluminium (50 µM) treatment. In the meanwhile the same concentration of the metal had induced phenylalanine ammonia-lyase activity by 2 times.

Keywords

• aluminium resistance
• buckwheat
• oxalic acid
• phenolic compounds
• phenylalanine ammonia-lyase