International Journal of General Medicine (May 2023)
Development and Validation of a Diagnostic Model Based on Hypoxia-Related Genes in Myocardial Infarction
Abstract
Ke Jiang,1,* Ling Kang,1,* Andong Jiang,2 Qiang Zhao1 1Department of Cardiovascular Medicine, The Second Affiliated Hospital of Shandong First Medical University, Tai’an, Shandong, People’s Republic of China; 2Medical Imaging Department, The Second Affiliated Hospital of Shandong First Medical University, Tai’an, Shandong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qiang Zhao, Department of Cardiovascular Medicine, The Second Affiliated Hospital of Shandong First Medical University, No. 366, Taishan Street, Tai’an, Shandong, 271000, People’s Republic of China, Tel +86-13583832661, Email [email protected]: Myocardial infarction (MI) is a common cardiovascular disease, and its underlying pathological mechanism remains unclear. We aimed to develop a diagnostic model to distinguish different subtypes of MI.Patients and Methods: The gene expression profiles of MI from the GEO database and hypoxia-related genes (HRGs) from MSigDB were downloaded. Then, the different MI subtypes based on HRGs were identified with unsupervised clustering. The difference of expression patterns and hypoxic-immune status among different subtypes of MI were investigated. The diagnostic model to distinguish the different subtypes of MI was developed and validated.Results: Based on HRGs, MI samples were divided into two subtypes, cluster A and cluster B. A total of 211 genes showed significant changes in expression between the two subtypes. Cluster A was characterized by high hypoxia status and low immunity status. Based on weighted gene co-expression network analysis, ROC analysis and LASSO regression algorithm, 5 genes were identified as potential diagnostic markers. Finally, a diagnostic model based on these 5 genes was established, which can distinguish the two subtypes well.Conclusion: The five hub genes, including ANKRD36, HLTF, KIF3A, OXCT1 and VPS13A, may be associated with the different subtypes of MI.Keywords: myocardial infarction, hypoxia, immune, diagnostic model
