Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices

Abstract

Summary: Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation.For complete details on the use and execution of this protocol, please refer to Chang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Biophysics
• Cell Biology
• Cancer
• Biotechnology and bioengineering