Preliminary Studies on Microsatellite Marker Analysis of Resistance to Common Bunt in several Wheat Genotypes (Triticum aestivum L.)

Abstract

In order to use markers for the selection of wheat resistance to the common bunt, there were tested certain wheat microsatellite markers (SSRs) on certain local accessions of bread wheat. A number of eleven SSR markers were tested on parental forms (five lines of bunt resistant wheat - Bt5, Bt8, Bt10, Bt11 genes and a number of eight cultivars and lines susceptible to common bunt. From the eleven tested markers, the Xgwm633 marker and Xgwm114 marker identified polymorphism on parental forms. Relying on co-segregation method, the Xgwm633 marker revealed a polymorphic fragment of 200 bp on the resistant wheat line 99419G4-1A/1-1 and a 230 bp fragment on the susceptible cultivar Glosa. So the fragment of 200 bp obtained with this marker could be assigned to some common bunt resistant genes. The Xgwm114 marker revealed a polymorphic fragment of 120 bp on resistant wheat line 00274G2-31, harboring the Bt11 resistant gene, and two fragments of 160 bp and 180 bp on susceptible cultivar Glosa. So the fragment of 120 bp obtained with this marker could be assigned to Bt11 resistant gene. That result was also confirmed by BSA (Bulked Segregant Analysis) method. The linkage relationship between markers and resistant genes was evaluated on the F2 populations resulted from the cross between 99419G4-1A/1-1 and 00274G2-31 the wheat resistant lines with the susceptible cultivar (Glosa). The value of recombination between the Xgwm633 marker and a common bunt resistant gene was evaluated at 32 cM. A smaller value of recombination has been estimated between the marker Xgwm114 and the resistant gene (8 cM).