Antimicrobial Peptide Arenicin-1 Derivative Ar-1-(C/A) as Complement System Modulator
Ilia A. Krenev,
Ekaterina S. Umnyakova,
Igor E. Eliseev,
Yaroslav A. Dubrovskii,
Nikolay P. Gorbunov,
Vladislav A. Pozolotin,
Alexei S. Komlev,
Pavel V. Panteleev,
Sergey V. Balandin,
Tatiana V. Ovchinnikova,
Olga V. Shamova,
Mikhail N. Berlov
Affiliations
Ilia A. Krenev
Department of General Pathology and Pathological Physiology, Institute of Experimental Medicine, Acad. Pavlov Str. 12, 197376 Saint Petersburg, Russia
Ekaterina S. Umnyakova
Department of General Pathology and Pathological Physiology, Institute of Experimental Medicine, Acad. Pavlov Str. 12, 197376 Saint Petersburg, Russia
Igor E. Eliseev
Nanobiotechnology Laboratory, Alferov University, Khlopin Str. 8/3, 194021 Saint Petersburg, Russia
Yaroslav A. Dubrovskii
Faculty of Chemistry, Saint Petersburg State University, Universitetskaya Emb, 7/9, 199034 Saint Petersburg, Russia
Nikolay P. Gorbunov
Department of General Pathology and Pathological Physiology, Institute of Experimental Medicine, Acad. Pavlov Str. 12, 197376 Saint Petersburg, Russia
Vladislav A. Pozolotin
Department of General Pathology and Pathological Physiology, Institute of Experimental Medicine, Acad. Pavlov Str. 12, 197376 Saint Petersburg, Russia
Alexei S. Komlev
Department of General Pathology and Pathological Physiology, Institute of Experimental Medicine, Acad. Pavlov Str. 12, 197376 Saint Petersburg, Russia
Pavel V. Panteleev
M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow, Russia
Sergey V. Balandin
M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow, Russia
Tatiana V. Ovchinnikova
M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997 Moscow, Russia
Olga V. Shamova
Department of General Pathology and Pathological Physiology, Institute of Experimental Medicine, Acad. Pavlov Str. 12, 197376 Saint Petersburg, Russia
Mikhail N. Berlov
Department of General Pathology and Pathological Physiology, Institute of Experimental Medicine, Acad. Pavlov Str. 12, 197376 Saint Petersburg, Russia
Antimicrobial peptides (AMPs) are not only cytotoxic towards host pathogens or cancer cells but also are able to act as immunomodulators. It was shown that some human and non-human AMPs can interact with complement proteins and thereby modulate complement activity. Thus, AMPs could be considered as the base for complement-targeted therapeutics development. Arenicins from the sea polychaete Arenicola marina, the classical example of peptides with a β-hairpin structure stabilized by a disulfide bond, were shown earlier to be among the most prospective regulators. Here, we investigate the link between arenicins’ structure and their antimicrobial, hemolytic and complement-modulating activities using the derivative Ar-1-(C/A) without a disulfide bond. Despite the absence of this bond, the peptide retains all important functional activities and also appears less hemolytic in comparison with the natural forms. These findings could help to investigate new complement drugs for regulation using arenicin derivatives.
