Formulation of Alternative Culture Media from Natural Plant Protein Sources for Cultivation of Different Bacteria and Fungi

Abstract

This study was carried out to find out some semisynthetic new media for routine microbiological tests in microbiology laboratories, with cheap natural protein source. Agar culturemedia were formulated using natural plant sources such as cowpea, lentil, split pea, chickpea, soy protein, mung beans and rice and used for cultivation of Staphylococcus aureus, Escherichia coli, B. cereus, Pseudomonas aeruginosa, Penicillium sp. and Aspergillus sp. All tested microorganisms grew well on most plant sources except on rice. B. cereus showed the best growth and large colonies on formulated media. E. coli and P. aeruginosa also showed good growth, and Pigmentation of P. aeruginosa was not affected. S. aureus, P. aeruginosa and B. cereus produced beta hemolysis on all formulated media. Each bacteria exhibit the same diameter of inhibition of antimicrobials used zone on all formulated media. Results showed that most protein sources used were suitable for cultivation, hemolysis detection and antimicrobial susceptibility test.

Keywords

• Alternative media; formulated; protein source; bacteria cultivation.