Ultrasound-Assisted Enzyme Extraction, Physicochemical Properties and Antioxidant Activity of Polysaccharides from <i>Cordyceps militaris</i> Solid Medium
Xiaoya Wang,
Jingyan Zhang,
Kang Zhang,
Zhiting Guo,
Guowei Xu,
Liping Huang,
Lei Wang,
Jianxi Li
Affiliations
Xiaoya Wang
Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China
Jingyan Zhang
Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China
Kang Zhang
Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China
Zhiting Guo
Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China
Guowei Xu
Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China
Liping Huang
Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China
Lei Wang
Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China
Jianxi Li
Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China
Cordyceps militaris solid medium polysaccharides (CMMPs) were extracted using an ultrasound-assisted enzyme method, and the process conditions were optimized via response surface methodology (RSM). The CMMPs were separated into four components named CMMP−1, CMMP−2, CMMP−3 and CMMP−4 using ethanol fractional precipitation, and their monosaccharide composition and structural properties were analyzed by molecular weight analysis, Fourier-transform infrared spectroscopy (FT–IR), scanning electron microscopy (SEM), Congo red test, ultraviolet–visible spectroscopy (UV-vis), atomic force microscopy (AFM), and thermogravimetric analysis (TGA). RSM could predict the yield of the CMMP (R2 = 0.9928), and the polysaccharide yield was 15.43% under the selected conditions of 3.1% cellulase enzyme addition, a liquid–solid ratio of 42:1, an extraction temperature of 61 °C, and an extraction time of 60 min. Glucose and galactose were the main constituents of the four fractional precipitated polysaccharides. Furthermore, four components exhibited antioxidant activity, and CMMP−1 demonstrated stronger antioxidant activity in vitro. This study demonstrates the possibility of developing a natural antioxidant food from Cordyceps militaris solid medium.
