Poljoprivreda (Sep 2015)

LACTATE DEHYDROGENASE GENE GENOTYPIZATION FOR SPECIES IDENTIFICATION IN A FISH FARM ON THE RIVER NERETVA

  • Polonca Margeta,
  • Ivan Bogut,
  • Marko Ivanković,
  • Vladimir Margeta

DOI
https://doi.org/10.18047/poljo.21.1.sup.12
Journal volume & issue
Vol. 21, no. 1 sup
pp. 56 – 58

Abstract

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There are severale Salmonid species, found in the river Neretva basin, among which S. trutta and S. obtusirostris. Also, natural hybrids such as S. obtusirostris x S. trutta have been observed. In one fish farm on the river Neretva, S. trutta and S. obtusirostris were decided to breed separately. Parental fishes were separated phenotypicaly on the basis of the morphological signs. PCR-RFLP analysis of the exon 3 to exon 4 part of the lactate dehydrogenase (LDH) C1* gene with restriction endonuclease RsaI was employed to identify the presence of other species representatives or intercrosses in two groups of juvenille fishes. Using this method, we were able to identify two S. trutta representatives in the S. obtusirostris group.

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