Real Time Monitoring of a UV Light-Assisted Biofunctionalization Protocol Using a Nanophotonic Biosensor
Jad Sabek,
Luis Torrijos-Morán,
Amadeu Griol,
Zeneida Díaz Betancor,
María-José Bañuls Polo,
Ángel Maquieira,
Jaime García-Rupérez
Affiliations
Jad Sabek
Nanophotonics Technology Center, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
Luis Torrijos-Morán
Nanophotonics Technology Center, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
Amadeu Griol
Nanophotonics Technology Center, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
Zeneida Díaz Betancor
Departamento de Química, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico IDM, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
María-José Bañuls Polo
Departamento de Química, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico IDM, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
Ángel Maquieira
Departamento de Química, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico IDM, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
Jaime García-Rupérez
Nanophotonics Technology Center, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain
A protocol for the covalent biofunctionalization of silicon-based biosensors using a UV light-induced thiol–ene coupling (TEC) reaction has been developed. This biofunctionalization approach has been used to immobilize half antibodies (hIgG), which have been obtained by means of a tris(2-carboxyethyl)phosphine (TCEP) reduction at the hinge region, to the surface of a vinyl-activated silicon-on-insulator (SOI) nanophotonic sensing chip. The response of the sensing structures within the nanophotonic chip was monitored in real time during the biofunctionalization process, which has allowed us to confirm that the bioconjugation of the thiol-terminated bioreceptors onto the vinyl-activated sensing surface is only initiated upon UV light photocatalysis.
