Bezmiâlem Science (Apr 2024)

Evaluation of The Effect of Different Polyetheretherketone Materials on Biofilm Formation: An in vitro Study

  • Sibel KAYAALTI-YÜKSEK,
  • Kevser ATALIK,
  • Begüm KARADEMİR,
  • Serkan SARIDAĞ,
  • Ayşe Demet KAYA,
  • Kübra DEĞİRMENCİ

DOI
https://doi.org/10.14235/bas.galenos.2023.93823
Journal volume & issue
Vol. 12, no. 2
pp. 191 – 197

Abstract

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Objective: The aim of this in vitro study was to investigate microorganism adhesion and biofilm formation between pure and ceramic-reinforced polyetheretherketone (PEEK) materials. Methods: A total of 72 rectangular (8 x 8 x 4 mm) samples were prepared from pure-PEEK without filler and PEEK (Ceramicreinforced PEEK - bio high-performance polymer) containing 20% nano-ceramic filler. A profilometer contact surface measurement device was used to assess the surface roughness of the samples. PEEK groups (36 pure PEEK, 36 Ceramic-reinforced PEEK) were divided into 4 sub-groups of 9 according to the microorganism strains. Staphylococcus aureus [American Type Culture Collection (ATCC 29213)], Acinetobacter baumannii (ATCC 19606), Enterococcus faecalis (ATCC 29212), and Candida albicans (ATCC 10231) standard strains were used for microbiological analysis. Blocks were added to 24-well microplates containing suspensions of microorganisms and were incubated at 37 °C for 72 hours. Microplates were read at a wavelength of 490 nm using crystal violet. Results: No significant difference was determined between the PEEK groups in terms of surface roughness. No significant differences in biofilm formation of S. aureus, A. baummanii, E. faecalis, and C. albicans strains were found between the PEEK groups (p>0.05). In the pure-PEEK, the highest adhesion was recorded in S. aureus (p<0.001), and the lowest adhesion in C. albicans (p<0.001). In the ceramic-reinforced PEEK group, S. aureus and A. baummanii adhesions were observed more than E. faecalis and C. Albicans (p<0.001). Conclusion: The results of this investigation demonstrated no significant differences in the biofilm formation of different strains between PEEK materials. This was a preliminary study to define the biological characteristics of ceramic-reinforced PEEK. There is a need for further comparative and clinical studies on this subject.

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