Frontiers in Genetics (Dec 2022)

Differential expression of cyclins CCNB1 and CCNG1 is involved in the chondrocyte damage of kashin-beck disease

  • Kaidiriye Setiwalidi,
  • Jialei Fu,
  • He Hei,
  • Shaniya Nuer,
  • Feiyu Zhang,
  • Sijie Chen,
  • Yanli Liu,
  • Feihong Chen,
  • Shujin Li,
  • Chaowei Wang,
  • Yifan Wu,
  • Yi Gong,
  • Minhan Hu,
  • Ruitian Huang,
  • Junyi Liu,
  • Tianxiao Zhang,
  • Yujie Ning,
  • Hongmou Zhao,
  • Xiong Guo,
  • Xiong Guo,
  • Xi Wang

DOI
https://doi.org/10.3389/fgene.2022.1053685
Journal volume & issue
Vol. 13

Abstract

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The purpose of this study was clarify the relationship between the differential expression of cyclins CCNB1 and CCNG1 and chondrocyte damage in Kashin-Beck disease. Systematic review and high-throughput sequencing of chondrocytes derived from Kashin-Beck disease patients were combined to identify the differentially expressed cyclins and cyclin-dependent kinase genes. In parallel, weaned SD rats were treated with low selenium for 4 weeks and then T-2 toxin for 4 weeks. Knee cartilage was collected to harvest chondrocytes for gene expression profiling. Finally, the protein expression levels of CCNB1 and CCNG1 were verified in knee cartilage tissue of Kashin-Beck disease patients and normal controls by immunohistochemical staining. The systematic review found 52 cartilage disease-related cyclins and cyclin-dependent kinase genes, 23 of which were coexpressed in Kashin-Beck disease, including 15 upregulated and 8 downregulated genes. Under the intervention of a low selenium diet and T-2 toxin exposure, CCNB1 (FC = 0.36) and CCNG1 (FC = 0.73) showed a downward expression trend in rat articular cartilage. Furthermore, compared to normal controls, CCNB1 protein in Kashin-Beck disease articular cartilage was 71.98% and 66.27% downregulated in the superficial and middle zones, respectively, and 12.06% upregulated in the deep zone. CCNG1 protein was 45.66% downregulated in the superficial zone and 12.19% and 9.13% upregulated in the middle and deep zones, respectively. The differential expression of cyclins CCNB1 and CCNG1 may be related to articular cartilage damage in Kashin-Beck disease.

Keywords